Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Journal of pharmacological and toxicological methods

Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models

doi: 10.1016/j.vascn.2022.107159

Figure Lengend Snippet: List of Used Material

Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic media preparation (MNCC) Mouse Neuronal Cell Culture Complete Growth Media with 10% Fetal Bovine Serum (FBS) Celprogen M11003-02S For maintenance of MNCC, used for normoxic conditions; also referred to as regular/normal media Mouse Neuronal Cell Culture Extracellular Matrix – 96 Black Wall Well Plates (5/pk) Celprogen E1 1003-02-96BW For fluorometric-based assays (cf.

Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: Adipomes play a major regulatory role in cardiomyocytes functioning (A–C) Fold change in mRNA transcript levels of genes involved in adipogenic and lipogenic signaling (A), mitochondrial oxidative phosphorylation (B), and inflammatory signaling (C) (normalized to Gapdh ) in murine primary cardiomyocytes treated with CLA, ILA, CSA, or ISA for 48 h and compared to untreated cardiomyocytes as demonstrated by qPCR analysis ( n = 3). All adipome-treated groups (CLA, ILA, CSA, and ISA) were compared to untreated groups. Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques:

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: Circulatory adipomes induce ER stress in cardiomyocytes Immunoblot analysis of β1-AR and CHOP expression in murine primary cardiomyocytes treated with plasma-derived adipomes (P-CLA, P-ILA, P-CSA, and P-ISA) and naive cells ( n = 3/group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to GDI. The “&” symbol denotes significance calculated between different adipome-treated groups. Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, and ∗∗∗ p ≤ 0.001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Western Blot, Expressing, Derivative Assay

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: P-ILA cause mitochondrial dysfunction in primary cardiomyocytes (A) Seahorse Mito Stress assay showing the oxygen consumption rate (OCR) from plasma-adipome treated (P-CLA and P-ILA) and untreated mouse cardiomyocytes ( n ≥ 6). Data normalized to nuclear content by staining and fluorescence cell counting. (B–F) Representative graphs depicting the different mitochondrial respiration parameters evaluated, such as basal respiration (B), maximal respiration (C), proton leak (D), spare respiratory capacity (E), and ATP production (F). Data are represented as mean ± SEM. (∗ p < 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗ p ≤ 0.0001).

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Staining, Fluorescence, Cell Counting

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet:

Article Snippet: Adult Mouse Cardiomyocytes were purchased from Celprogen and cultured as a monolayer in flasks pre-coated with Adult Mouse Cardiomyocyte Cell Culture Extra-cellular Matrix.

Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software

Journal: PLoS ONE

Article Title: Comprehensive In Vitro Toxicity Testing of a Panel of Representative Oxide Nanomaterials: First Steps towards an Intelligent Testing Strategy

doi: 10.1371/journal.pone.0127174

Figure Lengend Snippet: Target organs or systems for NMs in the present study: immune system (HMDM, RAW264.7 and MH-S), respiratory system (Calu-3, 16HBE and RLE-6TN), male reproductive system (TM3 and TM4), gastrointestinal system (Caco-2), kidneys (NRK-52E) and embryo (NIH-3T3 and mES/D3).

Article Snippet: Mouse NIH/3T3 cells and D3 mouse Embryonic Stem cells (mES) were purchased from ATCC (Manassas, VA, USA).

Techniques: